TABLE 2.

Analysis of 2-series variants by RNase protection assay and Northern blotting^a

DHBV	RNase protection assay			Northern blotting
	pgb	pre-S/Sc	Splicedd	pgb	sge
WT	1.00	0.10 ± 0.04	0.45 ± 0.08	1.00	0.69 ± 0.04
B2	0.09 ± 0.01	0.12 ± 0.04	0.96 ± 0.04	0.08 ± 0.02	1.25 ± 0.01
A2	0.07 ± 0.02	0.12 ± 0.05	1.01 ± 0.04	0.07 ± 0.02	1.31 ± 0.07
A2/B2	0.68 ± 0.13	0.39 ± 0.14	0.55 ± 0.05	0.73 ± 0.06	1.10 ± 0.10

^aValues are based on measurements made on poly(A)⁺ RNA isolated from independent transfections, with two for the wild type (WT) and B2, and four for A2 and A2/B2. First, all DHBV RNA levels were normalized to the level of GFP mRNA. Then, the value of WT pgRNA was set to 1.00 and all other RNA levels were accordingly adjusted, and then the mean and standard deviation were calculated.

^bPregenomic class of RNAs.

^cpre-S/S RNA.

^dSpliced RNA.

^eSubgenomic class of RNAs.