TABLE 3.

Analysis of 3-series variants by RNase protection assay and Northern blotting^a

	RNase protection assay		Northern blotting
	pgb	Splicedc	pgb	sgd
WT	1.00	0.36 ± 0.04	1.00	0.69 ± 0.07
B3	0.16 ± 0.05	0.82 ± 0.28	0.14 ± 0.02	1.07 ± 0.24
A3	0.14 ± 0.08	0.26 ± 0.15	0.09 ± 0.04	0.55 ± 0.22
A3/B3	0.36 ± 0.10	0.16 ± 0.07	0.25 ± 0.04	0.37 ± 0.07

^aValues are based on measurements made on poly(A)⁺ RNA isolated from independent transfections, with five for wild type (WT), four for A3/B3, and three for B3 and A3. First, all DHBV RNA levels were normalized to the level of GFP mRNA. Then, the value of WT pgRNA was set to 1.00 and all other RNA levels were accordingly adjusted, and then the mean and standard deviation were calculated.

^bPregenomic class of RNAs.

^cSpliced RNA.

^dSubgenomic class of RNAs.