FIG. 6.

Sensorgrams of binding of purified proteins to model P/T. Binding of Pol (A and D), UL42 (B and E), and Pol/UL42 heterodimer (C and F) to biosensor SA-gold chips to which 120 RU of biotinylated P/T was bound was measured using the BIAcore 2000 system as described in Materials and Methods. Curves of increasing thickness denote binding of 25, 50, 150, 300, and 600 nM protein. Binding and dissociation were performed in buffer A containing either 50 mM KCl (A to C) or 125 mM KCl (D to F). Sensorgrams shown are representative of at least two independent experiments for each protein and the concentrations indicated.