FIG. 3.

Semiquantitative RT-PCR analysis of normal (N-Mg) and CJD-infected (CJD-Mg) microglia, as well as neonatal normal astrocytes (N-Ast). Representative blots of biotinylated RT-PCR products for microglial markers and control transcripts (A), chemokines (B), and other inflammatory mediators (C). GFAP was also examined to confirm the absence of astrocytes in the microglial cultures, and GAPDH was always included as a standard for differences in RNA loading. Separate preparations of normal microglia were analyzed in parallel with astrocytes or CJD-infected microglia in each experiment.