FIG. 7.

Conventional micrographs of cells infected with PV M1 and S2. (a to e) FISH was performed with a FITC-labeled probe of 2,980 nt at different times p.i. (b to e) The images were superimposed on the corresponding phase-contrast images. Viral RNA is found at the cell periphery and in the cytoplasm at 0.5 (a and b) and 1 (c) h p.i. and in a juxtanuclear region at 1.5 h p.i. (d). The signals in panels a to d represent input viral RNA. Replication complexes start to form around 2.5 h p.i. (e), as judged from the appearance of PV vesicles, visualized in a parallel specimen by phase-contrast-IF with anti-2B MAb (red) (f). Bar, 10 μm.