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Growth of NCO cells in soft agara
| Cell line | No. of colonies at:
|
|
|---|---|---|
| Passage 2 | Passage 6 | |
| NCO | 0/0/0/0 | 0/0/0/0 |
| NCO/v | 0/0/0/0 | 0/0/0/0 |
| NCO/t | 60/55/50/71 | >200 |
| NCO/wt-Akt | 0/1/0/2 | 0/1/3/0 |
| NCO/ca-Akt | 0/1/0/0 | 0/0/0/0 |
| NCO/hTERT | 1/0/1/0 | 0/0/0/0 |
| NCO/wt-Akt+hTERT | 0/0/0/0 | 0/1/0/0 |
| NCO/ca-Akt+hTERT | 0/1/0/1 | 1/0/0/0 |
Petri dishes (35 mm) initially were seeded with 2 × 10 3 cells. Experiments were performed twice, each time in duplicate. Colonies were counted after 4 weeks.
