FIG. 5.

Expression of immunologically relevant molecules on HTNV-infected immature DCs and HUVECs. Cells were mock infected (infected with UV-inactivated virus), infected with HTNV (MOI = 1), or treated with TNF-α (1,000 U/ml) as indicated. After 3 (HUVECs) or 4 (DCs) days, the cells were analyzed by flow cytometry. (A) Levels of adhesion molecules on immature DCs (ICAM-1 and DC-SIGN) and HUVECs (ICAM-1 and VCAM-1). (B) Expression of costimulatory molecules on immature DCs (CD40, CD80, and CD86) and HUVECs (CD40). (C) Density of MHC class I and II molecules on immature DCs and HUVECs. The unfilled curves represent expression of the marker molecules as indicated, whereas the gray filled-in curves show staining of cells with an irrelevant antibody (isotype control). The x axis shows the fluorescence intensity (log scale, four decades), whereas the y axis depicts the relative cell number. The mean fluorescence intensity is given in the upper right corner of each histogram. The data are representative of three individual experiments.