FIG. 1.
Construction of recombinant viruses with deletions in ORF17. The VZV genome is 124,884 bp long (top) and consists of unique long (UL), unique short (US), terminal repeat (TR), and internal repeat (IR) DNA segments. NotI and MstII DNA restriction fragments used to construct parental VZV are diagrammed. Cosmid NotI A-17DA has codons 174 to 378 of ORF17 deleted, whereas cosmid NotI A-17DB has codons 90 to 304 deleted. Numbers in diagrams of cosmids NotI A-17DA and NotI A-17DB indicate the sites of the deletions. Cosmids NotI A-17DA and NotI A-17DB were used to construct viruses ROka17DA and ROka17DB, respectively.