FIG. 11.

Infection of cotton rats with cells containing ROka or ROka17DA results in ORF63 transcripts expressed in dorsal root ganglia. RNA was isolated from dorsal root ganglia, cDNA was amplified, and PCR was performed, followed by Southern blotting for ORF63. ORF63 transcripts were detected in animals 1, 2, 3, 5, 6, 8, and 9 infected with VZV ROka and in animals 1, 3, 4, 5, 6, and 9 infected with ROka17DA. Animals inoculated with uninfected cells or with cells containing heat-inactivated (HI) ROka had no detectable ORF63 transcripts. ORF63 RNA was present in VZV-infected cells. cDNA was prepared in the presence (+) or absence (−) of reverse transcriptase (RT).