FIG. 9.

ORF17 is responsible for normal distribution of ORF62 protein at 37°C. Melanoma cells were infected with cell-free VZV, incubated at 37 or 33°C for 48 h, fixed in cold acetone-methanol, and stained with an antibody to ORF62 protein (A through D) or gE (E through H). Cells were infected either with VZV ROka17DAR at 37°C (A and E) or 33°C (B and F) or with ROka17DA at 37°C (C and G) or 33°C (D and H). Melanoma cells were also infected with cell-free VZV, incubated at 33°C for 3 h followed by 37°C for 48 h, and stained with an antibody to ORF62 protein either alone (I and K) or with DAPI (J and L). Cells were infected with either VZV ROka17DAR (I and J) or ROka17DA (K and L). ORF62 protein shows nuclear-membrane and punctate nuclear staining in cells infected with ROka17DA at 33°C or with ROka17DAR at 33 or 37°C. ORF62 protein is present in coarse clumps in the nuclei and cytoplasm, but not in the nuclear membranes, of cells infected with ROka17DA at 33°C. Magnification, ×504 for panels A through H and ×320 for panels I through L.