FIG. 1.

IPC express HIV coreceptors. (a) Blood DC from healthy subjects were phenotypically defined by flow cytometry as expressing MHC-II and lacking lineage markers CD3, -14, -19, and -56 (box). (b) These gated cells were further divided into two distinct subsets either expressing high levels of CD123 and lacking CD11c (IPC) (solid box) or expressing CD11c (MDC) (dashed box). (c) IPC were then further characterized for their surface expression of CD4, CXCR4, CCR5, CD62L, and DC-SIGN. Open histograms represent cells stained with isotype-matched control antibodies. (d) The capacity of IPC (black bar) and MDC (white bar) to migrate in response to SDF-1α and MIP-1α was assessed in a transwell chemotaxis assay. Error bars represent the standard deviation for three replicate transwells. (e) Following 10 daily doses of Flt3L to expand DC in vivo, analysis of blood reveals that approximately 16% of PBMC are DC. (f) The proportion of IPC relative to MDC is essentially unchanged by Flt3L treatment. Data shown are representative of three experiments.