FIG. 5.

Identification of the ORF3 product. ORF3 RNA transcripts were translated in vitro in the absence or presence of canine pancreatic microsomes, and the expression products were immunoprecipitated with a GP₃-specific antipeptide serum (GP₃) or its preimmune serum (pGP₃). Subsequently, the precipitates were treated or mock treated with PNGase F or endo H. The samples were dissolved in LSB containing 5% β-mercaptoethanol and analyzed in SDS-15% PAA gels. The values on the left are the molecular sizes, in kilodaltons, of marker proteins analyzed in the same gel. Ab, antibody.