FIG. 1.

Expression and purification of recombinant p75-Fc protein in COS-7 cells. (A) Structural organization of the p75-Fc chimeric receptor, containing the four cysteine-rich domains and the O-glycosylated stalk from p75NTR fused to the Fc fragment of human IgG1. The chimera receptor was produced in the supernatant of transiently transfected COS-7 cells and analyzed by Western blotting with a rabbit polyclonal anti-p75NTR antibody (B). After purification on a protein A-Sepharose column, the proteins were analyzed under reducing (R) and nonreducing conditions (NR) by SDS-PAGE and Coomassie blue staining of the gel (C).