FIG. 4.

Interaction between the srr mutant glycoprotein and p75-Fc or anti-RVG MAbs. BSR cells were infected with the different srr mutants at MOIs of 2 to 5. (A) After 24 h, the cells were radiolabeled for 1 h and then solubilized in TD buffer containing 1% CHAPS. Cell extracts were immunoprecipitated with anti-RVG MAbs or with p75-Fc preadsorbed onto protein A-Sepharose (protA) and analyzed by SDS-PAGE before autoradiography. (B) The interaction was also analyzed by immunofluorescence of RV-infected cells 18 h postinfection. Cells were incubated with MAb 50AD1, fixed with paraformaldehyde, and then incubated with a fluorescein isothiocyanate-conjugated antimouse antibody. Magnification, ×50.