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Maturation of the srr mutant glycoproteins. BSR cells were infected with CVS or srr mutants (F318S and H352R) at an MOI of 5 for 20 h and then pulse-labeled for 3 min and chased for the indicated times. Cells were solubilized with TD buffer containing 1% CHAPS, and glycoproteins were immunoprecipitated with anti RVG MAb 17D2 before SDS-PAGE analysis and autoradiography.
