TABLE 1.
Measles virus MAbsa
| Hybridoma | Antigen specificity | Isotype | % Oligomeric IgA |
|---|---|---|---|
| 16CD11-G | H | IgG1 | 0 |
| 16DC9-G | F | IgG1 | 0 |
| 16CF7-G | N | IgG1, IgG2a | 0 |
| 16CD11-A | H | IgA | 73 |
| 16DC9-A | F | IgA | 62 |
| 16CF7-A | N | IgA | 70 |
a
The hybridomas were established by Sheshberadaran et al. (28). The specificities originally determined were confirmed in the present work by Western blotting after the switch to IgA. Antigens H and F are on the surface of the virus, and N is internal. IgA and IgG isotypes, including IgG subclasses, were determined by ELISA. Hybridoma 16CF7-G secreted both IgG1 and IgG2a. The percentage of the total IgA that was oligomeric was assessed by fast protein liquid chromatography.