TABLE 2.
Blocking by antibodies of apical measles virus infection of polarized epithelial cellsa
MAb (400 μg/ml) | Apical supernatant
|
Cell lysate
|
||
---|---|---|---|---|
Virus titer (10−2 PFU/ml) | % Reduction of virusb | Virus titer (10−4 PFU/ml) | % Reduction of virusb | |
Anti-H IgA | 0c | 100 | 0.68 ± 0.07c | 82 |
Anti-H IgG | 4.55 ± 0.70c | 41 | 3.20 ± 0.42d | 15 |
Anti-F IgA | 5.00 ± 0.28c | 35 | 2.78 ± 0.81d | 26 |
Anti-F IgG | 4.40 ± 0.57c | 43 | 3.20 ± 0.28d | 15 |
Anti-N IgA | 5.75 ± 0.35c | 26 | 3.55 ± 0.35d | 6 |
Anti-N IgG | 6.90 ± 0.57d | 11 | 3.57 ± 0.11d | 6 |
Irrelevant IgA | 8.10 ± 0.07 | 0 | 4.40 ± 0.14 | 0 |
None | 7.75 ± 0.35 | 0 | 3.78 ± 0.60 | 0 |
Experiments were repeated on at least three occasions with similar results. Results of a representative experiment are shown. Data are means ± standard deviations (n = 3).
Compared to no-antibody control.
Mean virus titers were significantly lower (P ≤ 0.002) than those from cells exposed to no antibody.
Mean virus titers were not significantly lower (P ≥ 0.05) than those from cells exposed to no antibody.