TABLE 3.
Intracellular virus neutralization by anti-H IgAa
| Cells and antibody (100 μg/ml) | Apical supernatant
|
Cell lysate
|
||
|---|---|---|---|---|
| Virus titer (10−4 PFU/ ml) | % Reduction of virusb | Virus titer (10−5 PFU/ ml) | % Reduction of virusb | |
| pIgR+ | ||||
| Anti-H IgA | 0.09 ± 0.02d | 97 | 0.49 ± 0.05d | 91 |
| Anti-H IgG | 3.09 ± 0.32e | 11 | 5.82 ± 0.36e | 0 |
| CMc | 1.72 ± 0.21d | 51 | 5.71 ± 0.19e | 0 |
| None | 3.48 ± 0.34 | 0 | 5.68 ± 0.39 | 0 |
| pIgR− | ||||
| Anti-H IgA | 2.85 ± 0.13d | 16 | 5.63 ± 0.24e | 9 |
| None | 3.41 ± 0.18 | 0 | 6.16 ± 0.25 | 0 |
a
Experiments were repeated on at least three occasions with similar results. Results of a representative experiment are shown. Data are means ± standard deviations (n = 3).
b
Compared to no-antibody control.
c
Conditioned medium (see the text).
d
Mean virus titers were significantly lower (P < 0.001) than those from cells exposed to no antibody.
e
Mean virus titers were not significantly lower (P ≥ 0.05) than those from cells exposed to no antibody.