FIG. 2.
(A) Transgene mRNA analysis. Shown are lacZ transgene mRNA levels as percentages of TBP mRNA levels in the kidney, liver, and spleen. Each circle represents one mouse. The diamond represents mRNA levels in a ROSA transgenic mouse, which expresses high, constitutive levels of lacZ mRNA and serves as a positive control. (B) Real-time RT-PCR analysis of lacZ (shaded squares) and apoB (solid squares) mRNA levels from heart tissue. Relative fluorescence (ΔRn) is recorded on a logarithmic scale on the vertical axis, and cycle number is shown on the horizontal axis. T indicates the threshold setting used for calculating individual Ct values. RT-negative samples showed no amplification.