FIG. 7.

CycT1 level is maintained during differentiation of monocyte-derived DC. Isolated monocytes were cultured under conditions to induce macrophage differentiation or differentiation to immature DC (iDC) or mature DC (mDC) as described in Materials and Methods. DC were treated with TNF-α to induce maturation. On the indicated days, cell extracts were prepared and CycT1, Cdk9, and β-actin protein levels were determined by immunoblotting. Similar results were obtained with DC from three additional donors.