FIG. 10.

Effect of phosphorylation by ORF47 kinase and CKII on gE localization. At the plasma membrane (PM), VZV gE associates with and is phosphorylated by either VZV ORF47 or CKII. VZV gE is internalized in early endosomes (EE). If VZV gE is phosphorylated on S593 or S595 (predominantly by ORF47), PACS-1 is postulated to bind the gE acidic cluster in the secondary endosomes (SE) and gE is transported to the TGN. If VZV gE is phosphorylated on T596 or T598 (half the time in ORF47-associated gE, predominantly in CKII-associated gE), PACS-1 is postulated to bind less well and gE recycles back to the plasma membrane.