FIG. 2.

Functional analysis of RSV P protein phosphorylation mutants. (A) MVA-T7-infected HEp-2 cells were transfected with pRSVCAT together with pN, pL, and wild-type (WT) or mutant pP plasmids. The CAT reporter gene activities were determined by CAT-ELISA and expressed as the percentage of that of wild-type P protein. Error bars represent the standard deviation of three replicate experiments. The serine substitution mutations are shown at the bottom of the graph. (B) Cells were cotransfected with wild-type pP in decreasing amounts together with increasing amounts of pP-DDD or pCITE vector, and the reporter gene activity was expressed as a percentage of that of wild-type P protein. (C) Northern blot analysis of transcription and replication of the RSVCAT/EGFP minigenome in cells expressing the indicated mutant P protein. The CAT mRNA and antigenomic RNA are indicated.