FIG. 5.

Immunoprecipitation of RSV-infected proteins infected with wild-type or phosphorylation mutants. Vero cells were infected with rA2, rA2-PP2, or rA2-PP5 at an MOI of 1.0 and incubated at 35°C. At 18 h of postinfection, proteins were radiolabeled with [³⁵S]Cys and [³⁵S]Met (100 μCi/ml) in DMEM deficient in cysteine and methionine or ³³P_i (100 μCi/ml) in DMEM deficient in phosphate for 4 h, immunoprecipitated either by anti-RSV polyclonal or by anti-P protein monoclonal antibodies, separated by SDS-PAGE (15% polyacrylamide), and autoradiographed. P indicates the mature form of the P protein, and P′ represents the immature form of P protein.