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US3 localizes to the Golgi apparatus. His16 cells were infected with 150 and 30 PFU of AdtetUS3 and Adtet-trans/cell, respectively. After 18 h, the cells were fixed and permeabilized and incubated simultaneously with rabbit antibodies to US3 (red) and mouse antibodies to cellular markers (green) PDI (A), p115 (B), TGN46 (C), or LAMP-1 (D). Primary antibodies were visualized with secondary fluorescent antibodies by using a laser scanning confocal microscope.
