FIG. 7.

Effects of US3 on cell surface class II molecules. His16 cells were infected with Adtet-trans alone (120 PFU/cell) or with AdtetUS3 and Adtet-trans (100 and 20 PFU/cell, respectively) for 18 h. Class II molecules on the surfaces of infected cells were stained by incubating suspended cells with L243 and then with fluorescein isothiocyanate-conjugated goat anti-mouse IgG. The results were assessed by flow cytometry. The designation of the histograms are as follows: shaded, secondary antibody control; dotted line, Adtet-trans-infected cells; thin line, AdtetUS2-infected cells; thick line, AdtetUS3-infected cells.