FIG. 9.

US3 reduces accumulation of DR-α/β complexes in lysosomal compartments. (A) Uninfected Neo6 cells were fractionated with Percoll gradients, and fractions were subjected to electrophoresis and Western blotting for LAMP-1, PDI, and DR-α. For DR-α, cells were infected either with 120 PFU of Adtet-trans/cell alone (DR/trans) or with 100 and 20 PFU of AdtetUS3 and Adtet-trans (DR/US3)/cell, respectively, before fractionation and Western blotting. (B) Neo6 cells were infected with 120 PFU of Adtet-trans/cell alone (trans) or with 100 and 20 PFU of AdtetUS3 and Adtet-trans (US3)/cell, respectively, for 18 h. Infected cells were radiolabeled for 1 h, and the label was chased for 8 h. The cells were fractionated with Percoll gradients, and the fractions were collected from the top and numbered 1 to 16. The membrane fractions were solubilized in NP-40-DOC lysis buffer, and DR proteins were immunoprecipitated with MAb DA6.147. The samples were subjected to electrophoresis and phosphorimager analysis.