FIG. 3.

Detection of proximal (E1A) and distal (Fiber) regions of the adenovirus genome in tonsil and adenoid lymphocytes. (A) Relative location of E1A, hexon, and fiber genes within the adenovirus genome. (B) Nested PCR for adenovirus E1A DNA (172-bp product). (C) Nested PCR for adenovirus fiber DNA (175-bp product). Samples from all patients shown were positive for hexon by real-time PCR, except for the sample from patient 31, which was negative. Nested PCR was performed on DNA purified from Ficoll-purified lymphocytes. +, positive control using either 50 or 5 copies of Ad2 DNA as template; −, negative water control.