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Cellular source of intrahepatic IFN-γ. IHLs harvested from HBV transgenic mice at the indicated time after IL-18 were surface stained with anti-CD3-FITC and anti-NK1.1-PE MAb and fixed in 2% paraformaldehyde. After fixation, cells were permeabilized, stained with anti-mouse IFN-γ-APC or isotype control-APC and analyzed by flow cytometry.
