TABLE 2.

Biochemical characterization of in vitro-assembled CLPs and in vivo-assembled nucleocapsid cores

Property	Cytoplasmic nucleocapsid cores or CLPs	Assay methoda,b
Ionic strength	Unstable in assembly buffer plus 0.5 M NaCl	Agarose gel, EM
pH	Stable between pH 5.2 and 8.2	Agarose gel, EM, gra- dient sedimentation
Temperature	Unstable above 55°C	Agarose gel
Nuclease sensitivity	Yes	Agarose gel, EM
Particle size	≈410 ± 10 Å	CryoEM

^aAll assays were performed as described by Tellinghuisen et al. (22).

^bEM refers to negative-stain EM.