Figure 3.

Further upregulation of HO-1 by hemin inhibits stasis and HO-1 inhibition by SnPP exacerbates stasis in sickle mice. S+S-Antilles (A) and BERK (B) mice with an implanted DSFC were untreated, injected with hemin (40 μmol/kg/d, i.p.) for 3 days, or injected with SnPP (40 μmol/kg/d, i.p.) for 3 days. Twenty-four hours after the third injection, stasis was measured after 1 hour of hypoxia (7% O₂/93% N₂) and 1 hour and 4 hours of reoxygenation in room air. n = 3–10 mice and a minimum of 20 venules per mouse. *P < 0.05, untreated versus hemin or SnPP. The proportions of venules exhibiting stasis at each time point were compared using a z test.