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Energy profiles for a Cl− ion when the Glu-148 side chain is extended toward the external vestibule, assuming dielectric constant of 35 for water in the pore. (Solid line) Protonated side chain, with a second Cl− ion at z = −3 Å. (Dashed line) unprotonated side chain, with a second ion at z = −6 Å (reproduced from Fig. 3).
