Skip to main content
. 2002 Nov;76(21):11054–11064. doi: 10.1128/JVI.76.21.11054-11064.2002

FIG. 5.

FIG. 5.

Us11C does not prevent PACT binding to PKR. Shown are the effects of increasing concentrations of purified Us11C on wt PACT interaction with PKR. PKR was immunoprecipitated from an extract of HT1080 cells treated with beta interferon by using PKR monoclonal antibody/protein G-Sepharose. The final two washes of immobilized PKR were done with a low-salt buffer. Us11C and then PACT were added to the immobilized PKR, followed by incubation for 15 min and then extensive washing with buffer. PACT interaction with PKR was analyzed by Western blotting using a domain 3-specific PACT antibody. All lanes contained PKR and lanes 2 to 5 contained 1 μg of PACT. In addition, 0.3 (lane 3), 1.0 (lane 4), and 2.4 (lane 5) μg of Us11C were added. The bottom panel shows the stripped Western blot reprobed with anti-PKR antibody.