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Us11 inhibits PACTΔ1-mediated apoptosis. TUNEL and immunofluorescence assays were performed with HT1080 cells transfected with PACTΔ1 alone (top four panels) or cotransfected with Us11 and PACTΔ1 (bottom four panels). (A) Phase-contrast micrographs; (B) DAPI staining, showing nuclei; (C) TUNEL staining, showing green-fluorescing cells undergoing apoptosis; (D) PACT-expressing cells revealed by their red immunofluorescence.
