TABLE 2.

Activation parameters for NaChBac and selected mutants

Construct	Activation midpoint, Vh-a (mV)	Apparent valence, z
WT	−35 ± 2.2 (7)	4.8 ± 0.6 (7)
DimerBL	−32 ± 0.5 (4)	4.8 ± 0.7 (4)
Tetramer	−35 ± 3.4 (6)	3.9 ± 0.6 (5)
195E	−22 ± 2.5 (7)	2.4 ± 0.1 (7)
195D	−21 ± 3.2 (5)	2.1 ± 0.1 (4)
192E	−15 ± 3.4 (3)	3.1 ± 1.3 (3)

Activation parameters were obtained from separate Ohm's Law/Boltzmann fits of the current-voltage curves for individual experiments, as described in Methods. Indicated values are the mean ± SEM, with the number of experiments indicated as (n).