Skip to main content
. 2005 Apr 15;89(1):266–273. doi: 10.1529/biophysj.104.052092

FIGURE 7.

FIGURE 7

AFM images of lipid films transferred to mica substrates at π = 40 mN/m. (A) 0 wt % dSP-B1-25. With no dSP-B1-25, the solid phase is smooth and slightly higher (∼0.5 nm) than the fluid phase, which is mottled light and dark gray. (B) 5 wt % dSP-B1-25. As is the case for SP-C, SP-B is located in the fluid phase domains. The solid phase domains remain smooth and are free of protrusion. dSP-B1-25 leads to the formation of isolated small protrusions from the fluid phase domains, with heights ranging from ∼5−10 nm. SP-B removes fluid phase lipids from the monolayer on compression, but not nearly as efficiently as SP-C. (C) 10 wt % dSP-B1-25. Increasing the dSP-B1-25 to 10 wt % increases the lateral dimensions of the protrusions but not their density. dSP-B1-25 removes material from the fluid phase, as does SP-C, but the location and distribution of the material is quite different, leading to a smaller effect on the surface viscosity.