FIG. 4.

Binding of a truncated form of gD, gD(285t), to HveA proteins expressed on transfected cells. Transiently transfected B78-H1 cells expressing wild-type HveA and the HveA mutants were seeded on 96-well plates, incubated with different concentrations of gDt, washed, and probed with an anti-gD PAb to detect the level of gDt binding. (A) Sample data are shown for wild-type HveA (wtHveA) and three representative HveA mutants. Assays detecting HveA expression were run in parallel (Fig. 3). (B) Data for all of the HveA mutant proteins binding gDt at one concentration, 100 nM gD(285t), are shown to illustrate the basis for the separation of the HveA mutants into reduced (striped bars), wild-type (black bars), and increased (crosshatched bars) binding categories. gDt binding is expressed as a percentage of gDt binding to wild-type HveA. Results from one representative experiment are shown. (C) Data for HveA mutants binding gDt at a higher concentration, 1 μM gD(285t), are shown to illustrate the basis for the separation of the HveA mutants into negative (white bars) and reduced (striped bars) categories.