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. 2005 May 6;89(1):402–412. doi: 10.1529/biophysj.104.058230

FIGURE 5.

FIGURE 5

pH dependence of the time constants obtained from the adduct decay in the LOV2 domain at 10 and 100 mM NaCl concentration and 100 mM phosphate buffer. (A) The two time constants of the biexponential decay in the wild-type LOV2 are plotted separately. The fitted curves are derived from a simple acid/base model (16) and yield an estimated pKa between 5 and 6. (B) In the LOV1/2-C57S mutant, only the LOV2 domain is forming the adduct. The slow component shown in A with its strong pH dependence is not present any more.