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Time constants of the adduct decay in the single and double LOV domains
| Species | Time constant τ1 (s) | Time constant τ2 (s) |
|---|---|---|
| LOV1 | 45 (15%) | 320 (85%) |
| LOV2 | 30 (25%) | 270 (75%) |
| LOV1/2 | 25 (30%) | 270 (70%) |
| LOV1/2-C250S | — | 260 |
| LOV1/2-C57S | 30 | — |
All samples were measured at pH 8, 10 mM NaCl, and 20°C. All domains were obtained from a Hisp2x construct.
