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Influence of cations and anions on the time constants of the adduct decay in LOV1
| Cation varied | Time constant (s) | Anion varied | Time constant (s) | ||
|---|---|---|---|---|---|
| Na+ | Cl− | 692 | Na+ | Cl− | 692 |
| NMG+ | Cl− | 659 | Na+ |  |
397 |
| K+ | Cl− | 645 | Na+ |  |
341 |
| (Et)4N+ | Cl− | 622 | |||
All samples were measured at pH 4.7, 10 mM phosphate buffer and 100 mM ion concentration. The LOV1 domain was obtained from a His-pMal construct.
