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1-PB binding to P450eryF mutants and CPM-labeled enzyme: wild-type (○), S93C (•), C154S (▿), C154A (▾), S93C/C154S (□), S93C/C154A (▪), and CPM-labeled S93C/C154S mutant (P450eryF-CPM, ⋄). Conditions: 0.1 M Na-Hepes buffer, pH 7.4, 1 mM DTT, 1 mM EDTA, and 2 μM enzyme, 25°C.
