TABLE 1.
Parameters of the interactions of P450eryF and its mutants with 1-PB
| P450eryF variant | S50, μM | Hill coefficient (n) | ΔFh (%) | Kion (mM) | Kspin(0) |
|---|---|---|---|---|---|
| Wild-type (S93/C154) | 7.9 ± 1.1 | 2.1 ± 0.2 | 29 ± 5 | 850 | 0.25 |
| C154I | 13.2 ± 1.8 | 1.4 ± 0.2 | 21 ± 4 | 1590 | 0.12 |
| C154A | 9.7 ± 0.3 | 2.3 ± 0.1 | 18 ± 2 | 1140 | 0.19 |
| C154T | 9.2 ± 0.1 | 1.8 ± 0.1 | 21 ± 3 | 1100 | 0.52 |
| C154S | 7.6 ± 0.7 | 1.5 ± 0.2 | 57 ± 3 | 340 | 0.75 |
| S93C | 10.0 ± 1.3 | 2.2 ± 0.2 | 16 ± 2 | 1190 | 0.14 |
| S93C/C154S | 8.4 ± 0.7 | 2.2 ± 0.2 | 41 ± 1 | 450 | 0.81 |
| S93C/C154A | 12.1 ± 0.2 | 2.4 ± 0.4 | 9 ± 1 | 2470 | 0.26 |
| S93C-CPM /C154S | 8.4 ± 0.5 | 2.4 ± 0.1 | 30 ± 6 | 600 | 0.61 |
| S93C-MDCC /C154S | 10.2 ± 1.0 | 1.7 ± 0.3 | 31 ± 3 | 1060 | 0.48 |
| S93C-bimane/C154S | 9.3 ± 2.0 | 2.3 ± 0.2 | 36 ± 2 | 350 | 0.54 |
The parameters of 1-PB binding represent the averages of four individual measurements plus/minus confidence intervals calculated for P = 0.95. Parameters of ionic strength dependence represent the results of a single measurement.