TABLE 1.
Kinetic parameters for M rise and decay for both native bR and bbR in DDW
| Sample | τ1 (A1) | τ2 (A2) | M rise τavg | τ1 (A1) | τ2 (A2) | M decay τavg |
|---|---|---|---|---|---|---|
| Native bR in DDW | 16 μs (0.09) | 90 μs (0.91) | 83 μs | 6 ms (0.72) | 20 ms (0.28) | 10 ms |
| Bicelle crystals in DDW | 3 μs (0.99) | 24 μs (0.01) | 24 μs | 73 ms | ——— | 73 ms |
| Native bR in DDW* | 8.49 μs (0.21) | 75.8 μs (0.79) | 61.7 μs | 8.57 ms | ——— | 8.57 ms |
| Native + CHAPS in DDW* | 23.5 μs (0.49) | 137 μs (0.51) | 81.4 μs | 27.7 ms | ——— | 27.7 ms |
| Native + Trition in DDW* | 1.76 μs (0.42) | 9.71 μs (0.58) | 6.71 μs | 9.47 ms (0.96) | 34.0 ms (0.04) | 10.5 ms |
τavg is calculated by τavg = (A1 × τ1) + (A2 × τ2), where A1 + A2 = 1. Data are presented as a time component (τ) with the relative contribution to the overall process (A) in parentheses.
*
From Heyes and El-Sayed (28).