TABLE 2.

Summary of unfolding statistics obtained for (protein L)₅ in phosphate-buffered saline at room temperature

Speed/nms−1	N*	Hit rate/%†	≥4 peaks/%‡	Mode Force/pN	Average (±SE)/pN	Mode interpeak distance (±SD)/nm	Average interpeak distance (±SE)/nm	Average ΔLc (±SE)/nm
40	116	18.6	78.6	86		15.6 (2.0)
40	55	5.7	60	92	91 (3)	14.7 (2.0)	15.2 (0.3)	N.D.
40	70	8.1	72	95		15.4 (1.7)
77	114	6.5	40	113		16.4 (1.5)
77	80	4.5	66.7	100	104 (4)	16.3 (1.4)	16.3 (0.1)	18.5 (0.1)
77	45	4	33.3	101		16.3 (1.6)
140	86	7.4	54.2	116		16.6 (1.8)
140	89	7.6	34.4	138	122 (8)	16.4 (1.3)	16.3 (0.3)	18.8 (0.2)
140	86	3.8	39.3	112		16 (1.9)
230	113	7.9	51.5	131		16.3 (3.2)
230	133	13.1	61.1	121	125 (3)	16.2 (1.7)	16.3 (0.2)	18.7 (0.1)
230	127	5.3	47.4	123		16.5 (1.5)
400	158	9.7	61.9	135		16.4 (1.5)
400	132	4.4	55.3	138	136 (1)	16.6 (1.6)	16.5 (0.1)	18.6 (0.1)
400	153	7.9	39.1	136		16.4 (1.6)
700	275	7.5	28.9	162		16.5 (1.6)
700	87	3.3	27.6	144	152 (5)	17.1 (1.4)	16.9 (0.2)	19.0 (0.1)
700	105	6.8	53.3	150		17.1 (1.6)
1400	131	8.1	60	167		16.6 (1.1)
1400	135	6.6	66.7	165	166 (1)	16.7 (1.7)	16.6 (0.1)	18.7 (0.1)
1400	113	5.1	47.1	167		16.6 (1.6)
2100	134	5.1	35.7	179		16.4 (1.4)
2100	107	3.4	48.3	175	179 (3)	17.3 (1.7)	16.9 (0.3)	19.0 (0.1)
2100	145	5.3	52.5	184		16.9 (1.4)
4000	104	9.6	31.4	208		16.9 (1.3)
4000	140	3.3	79.4	207	205 (3)	17.2 (2.1)	16.8 (0.2)	N.D.
4000	95	3	32.2	199		16.4 (1.1)

^*Number of unfolding events in data set.

^†Hit rate defined as percentage of the total number of force-extension profiles that remain after filtering data.

^‡Percentage of force-extension profiles after data filtering that contain four or more single protein L domain-unfolding events.