FIGURE 11.

Analysis of experimental data from the sedimentation of a natural killer cell receptor Ly49C (31 kDa) interacting with a MHC molecules H-2Kb (45 kDa) sedimenting at 50,000 rpm (35). The experimental parameters and best-fit sedimentation parameters from Lamm equation modeling (26) are equivalent to those simulated in Fig. 10. Panel A shows s_fast (circles) and s_w (squares), respectively, as determined from integration of c(s) (symbols). The red lines indicate the best-fit GJT isotherms with a 2:1 model, the blue lines the best-fit isotherms from an impostor single-site model.