TABLE 1.

Kinetic parameters of inactivation at 0 mV

	Whole oocytes		Outside-out patch
	Control	0.5 μM κ−PVIIA	Control	0.5 μM κ−PVIIA
Time constant(s)	4.5 ± 0.9	4.6 ± 0.5	1.44 ± 0.11	1.32 ± 0.13
Fractional steady state	0.21 ± 0.04	0.25 ± 0.04	0.11 ± 0.011	0.15 ± 0.012
n	12	8	10	7

Summary of the single exponential fits to the current decay in the absence and in the presence of 0.5 μM κ−PVIIA applied to the bath. The first 200 ms into the 0 mV pulse were excluded because at this time, the toxin has not yet reached equilibrium in either TEVC or in OOPC (see Fig. 2). In whole oocytes experiments, fits included ∼14 s into the trace, whereas in OOPC, the fits only includes 7 s subsequent to the first 200 ms. The fractional steady-state currents were calculated from the ratio of the current measured at 15 s divided by the current measured at 200 ms. n represents the number of oocytes or excised patches for TEVC and OOPC, respectively.