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BZLF1 enhances p53-specific DNA binding activity. A549 cells were infected with adenovirus vectors expressing LacZ, BZLF1, and p53. Whole-cell extracts were prepared at 48 h postinfection, and the level of p53-specific DNA binding activity was quantitated by EMSA analysis as described in Materials and Methods. The Pab421 antibody (421 Ab) has been shown to enhance p53 sequence-specific DNA binding activity.
