. 2005 May 27;89(2):1120–1131. doi: 10.1529/biophysj.105.059857

TABLE 2.

Assignment of carbon and proton chemical shifts for ergosterol inserted in a 8.4:1.6 (mol/mol) DMPC-d54/ergosterol lipid mixture

Carbon number	¹³C-chemical shift (ppm)	¹H-chemical shift (ppm)	Carbon number	¹³C-chemical shift (ppm)	¹H-chemical shift (ppm)
1	38.32	1.28^a/1.8^e	15	23.19	1.68
2	31.15	1.52^a/1.86^e	16	28.29	1.28/1.72
3	69.11	3.55	17	55.67	1.25
4	40.06	2.25/2.49	18	12.02	0.66
5	141.18	—	19	15.65	0.91
6	119.05	5.5	20	40.97	2.009
7	117.05	5.27	21	21.51	1.02
8	139.20	—	22	135.71	5.065
9	45.97	1.91	23	131.84	5.1
10	36.97	—	24	43.23	1.8
11	20.81	1.64	25	32.85	1.38
12	39.35	1.32^a/2.018^e	26	17.76	0.89
13	42.48	—	27	19.44	0.79
14	54.41	1.9	28	21.51	0.79

Frequencies in the proton and carbon dimensions are given with respect to the methyl 18. C₁₈ was set to12.08 ppm and H₁₈ at 0.66 ppm with respect to tetramethylsilane (TMS) by analogy with a liquid-state spectrum recorded in deuterated chloroform at 295 K. Errors on the reported chemical shifts are estimated to be ∼±0.02 ppm. (The expressions a and e correspond to the stereospecific assignment of methylene protons, i.e., a, axial; e, equatorial.)