FIG. 5.

Immunofluorescence microscopy analysis of the subcellular localization of HBZ with an N-terminal GFP tag in vivo. COS7 cells were transfected with expression vectors encoding either GFP-HBZ (A and B), GFP (C), or GFP-HBZΔbZIP (D). Cells were cultivated on the glass slides, fixed, and stained with Hoechst solution, and then the green fluorescence was analyzed by immunofluorescence microscopy. The specificity of the immunofluorescent staining is indicated by the absence of signal in flanking untransfected cells. The blue fluorescence of the nuclei (Hoechst staining) is visualized by UV illumination.