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. 2002 Dec;76(24):12813–12822. doi: 10.1128/JVI.76.24.12813-12822.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 6. — HBZ interacts with CREB-2. (A) In vitro binding assays of HBZ to CREB-2. Equal amounts of GST, GST-CREB-2, GST-CREB-2_263-351 (lane GST-bZIP), and GST-CREB-2_1-262 (lane GST-ΔbZIP) immobilized on glutathione-Sepharose beads were incubated with [³⁵S]HBZ, and bound proteins were analyzed by SDS-PAGE and autoradiography. The first lane corresponds to in vitro-translated [³⁵S]HBZ. (B) Binding of HBZ to CREB-2 in vivo. Proteins from MT4, MT2, C8166, CEM, and Jurkat cell total lysate were immunoprecipitated with rabbit anti-HBZ (I.P. αHBZ), and immunoprecipitated proteins were analyzed by immunoblotting with goat anti-CREB-2 (I.B. αCREB-2).