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HBZ abolishes the binding of CREB-2 to the CRE and TxRE sites. Biotinylated oligonucleotides (100 ng) corresponding to the somatostatin CRE were incubated with 50 ng of CREB-2 in the absence (lane 2) or the presence (lanes 3 and 4) of the bZIP domain of HBZ (25 and 50 ng). The same experiment was performed with the HTLV-1 TxRE III, CREB-2, and Tax (50 ng) in the absence (lane 6) or the presence (lanes 7 and 8) of HBZ bZIP.
